Spot Shape Scanning Device

Spot Shape Scanning Device

The UGA-42 GEO is a galvanometer based device, designed to generate light spots of different sizes and shapes in the field of view of the microscope. The device offers a variety of spot shapes and sizes to choose from, partly eliminating the need to scan in applications requiring the illumination of larger regions of interest (ROI), for example whole cells. Standard shapes include circles, squares, rectangles and donuts. The spot can be positioned anywhere in the field of view in under 1 ms. 

The UGA-42 GEO includes the scanner module, the optical and mechanical components necessary to create the spot, the microcontroller and the software. The optical module is coupled to the microscope without interfering with most other existing devices (e.g. spinning disk, LSM, etc.). The device uses lasers as light sources. The lasers are application specific and are ordered separately. 



Scanner module

  • Add-on module, can be integrated into existing setups
  • Two connections for fiber coupled lasers, one for large spot generation and one for point spots
  • Compatible with laser combiners
  • Switching time between any two spot locations <1ms
  • Scanning speed up to 5kHz
  • Multiple choices of preset shapes and sizes. Customization available upon request.
  • Wide variety of UV, VIS and NIR lasers available (266 - 1470 nm)
  • Switching time between two laser lines: <1ms.
  • Can be combined with wide field fluorescence, LSM and spinning disc confocal, etc.
  • Available for most modern research microscope brands and models




Software
 

  • UGA-40-GEO software runs in parallel with any other imaging or electrophysiology software
  • Simultaneous and independent spot illumination and imaging data acquisition
  • “Click & Fire” mode for real time spot illumination
  • Programmable illumination of multiple user defined locations and/or regions of interests (ROIs)
  • Spot geometries include circles, squares, rectangles and donuts
  • ROI geometries include: lines, circles, polygons, free-hand drawn shapes
  • Grid stimulation with sequential or random illumination of grid points
  • Two TTL inputs and two TTL outputs
  • TTL-triggered sequences 
  • TTL-triggered events within a sequence
  • Able to run multiple lasers within the same experiment




Applications
 

  • Optogenetics
  • Photoactivation/photoinhibition
  • Photoswitching
  • Photoconvertion
  • Photostimulation
  • Photobleaching and FRAP
  • Neural mapping, dynamics and plasticity





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