The QuVi SPIM is the latest product in LUXENDO’s range of light-sheet microscopes. Combining dual views with dual detection channels, it enables large-scale imaging of living samples.
Its novel stage design allows using SBS-format plates and provides an easy sample accessibility by means of its quick load feature. Since the objective lenses are exchangeable, a wide variety of samples of different sizes and preparations can be imaged.
Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.
High-precision XYZ stage
Unique quick-load feature, SBS plate compatible
Easy access from above for sample mounting, injections, etc.
Environmental control unit is optinally available, featuring precise control of temperature (range 20–37°C), humidity, O2 and CO2.
The QuVi SPIM's compact, vibration-free and robust design provides maximal stability during your long-term experiments.
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration compensation mechanism as all moving components are light-weighted and balanced with the motor technology. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.
The two spectral detection channels equipped with 10 position high-speed filter wheels and a dichroic mirror changer enable simultaneous dual channel imaging. In addition, more than two channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification and speed.
Compared to other techniques (e.g. confocal laser scanning and spinning disc microscopy) more time points can be acquired for a deeper insight into dynamic biological processes and fast movement tracking while avoiding photodamaging effects, the downside of increased illumination in those techniques.